Part:BBa_K3258033:Design

MDV + Qbeta + Spinach Aptamer Plasmid

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal prefix found in sequence at 5342
  Illegal suffix found in sequence at 2309
  Illegal XbaI site found at 180
  Illegal XbaI site found at 2231
  Illegal SpeI site found at 2107
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 5342
  Illegal NheI site found at 146
  Illegal SpeI site found at 2107
  Illegal SpeI site found at 2310
  Illegal PstI site found at 2324
  Illegal NotI site found at 2213
  Illegal NotI site found at 2317
  Illegal NotI site found at 5348
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 5342
  Illegal BglII site found at 172
  Illegal BglII site found at 2228
  Illegal BamHI site found at 1538
  Illegal BamHI site found at 1681
  Illegal XhoI site found at 168
  Illegal XhoI site found at 1212
  Illegal XhoI site found at 2222
  Illegal XhoI site found at 3343
  Illegal XhoI site found at 4235
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found in sequence at 5342
  Illegal suffix found in sequence at 2310
  Illegal XbaI site found at 180
  Illegal XbaI site found at 2231
  Illegal SpeI site found at 2107
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found in sequence at 5342
  Illegal XbaI site found at 180
  Illegal XbaI site found at 2231
  Illegal XbaI site found at 5357
  Illegal SpeI site found at 2107
  Illegal SpeI site found at 2310
  Illegal PstI site found at 2324
  Illegal NgoMIV site found at 2120
  Illegal NgoMIV site found at 2149
  Illegal AgeI site found at 1694
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 4381
  

Design Notes

Designed to demonstrate Qbeta self replication, as a result of MDV regions upstream and downstream, as well as the spinach aptamer for visualization.

Source

Synthetic construct.